Thomas M. Suszynski




     Year Entered: 2006

     Degrees Received:
     University of Minnesota
     Biomedical Engineering major
     B.Bm.En., 2006

     University of Minnesota
     Biomedical Engineering Graduate Program
     Ph.D., 2012

     Thesis Advisor: Klearchos Papas, Ph.D.

     Thesis Research : Islet transplantation has exhibited therapeutic
     promise in reversing clinical diabetes, but has yet to reach widespread acceptance and utility. One of the major limitations in achieving better outcomes is the poor control over and assessment of the quality of islet preparations prior to transplant. Additionally, it is unclear whether infusion of an islet suspension into the portal vein is the most optimal approach for the transplantation of these grafts. The studies herein aim to develop methods for both improved islet quality as well as improved quality assessment, with an emphasis on developing a non-invasive method based on fluorine magnetic resonance spectroscopy (19F-MRS) that enables real-time, prospective and quantitative characterization of the viability of tissue-engineered islet grafts prior to their eventual transplantation at an extrahepatic site. The technique is based on measuring the average oxygen partial pressure (pO2) within a tissue-engineered graft, and using diffusion-reaction modeling to calculate the fractional viability (based on the oxygen consumption rate [OCR] normalized per DNA content, or OCR/DNA) the tissue seeded into the engineered scaffold. Our laboratory has developed methods for the direct measurement of OCR/DNA in cell and islet suspensions, and these measurements will be corroborated with calculations of OCR/DNA derived of 19F-MRS and subsequent mathematical analysis.

Publications (pubmed):

Suszynski TM, Rizzari MD, Scott WE 3rd, Tempelman LA, Taylor MJ, Papas KK. Persufflation (or gaseous oxygen perfusion) as a method of organ preservation. Cryobiology. 2012 Jun;64(3):125-43.

Rizzari MD, Suszynski TM, Gillingham KJ, Dunn TB, Ibrahim HN, Payne WD, Chinnakotla S, Finger EB, Sutherland DE, Kandaswamy R, Najarian JS, Pruett TL, Kukla A, Spong R, Matas AJ. Ten-year outcome after rapid discontinuation of prednisone in adult primary kidney transplantation. Clin J Am Soc Nephrol. 2012 Mar;7(3):494-503.

Rizzari MD, Suszynski TM, Gillingham KJ, Matas AJ, Ibrahim HN. Outcome of living kidney donors left with multiple renal arteries. Clin Transplant. 2012 Jan-Feb;26(1):E7-11.

Suszynski TM, Avgoustiniatos ES, Stein SA, Falde EJ, Hammer BE, Papas KK. Assessment of tissue-engineered islet graft viability by fluorine magnetic resonance spectroscopy. Transplant Proc. 2011 Nov;43(9):3221-5.

Suszynski TM, Rizzari MD, Kidder LS, Mueller K, Chapman CS, Kitzmann JP, Pongratz RL, Cline GW, Todd PW, Kennedy DJ, O'Brien TD, Avgoustiniatos ES, Schuurman HJ, Papas KK. Paramagnetic microparticles do not elicit islet cytotoxicity with co-culture or host immune reactivity after implantation. Xenotransplantation. 2011 Jul-Aug;18(4):239-44.

Rizzari MD, Suszynski TM, Gillingham KJ, Matas AJ. Consideration of donor age and human leukocyte antigen matching in the setting of multiple potential living kidney donors. Transplantation. 2011 Jul 15;92(1):70-5.

Rizzari MD, Suszynski TM, Kidder LS, Stein SA, O'Brien TD, Sajja VS, Scott WE 3rd, Kirchner VA, Weegman BP, Avgoustiniatos ES, Todd PW, Kennedy DJ, Hammer BE, Sutherland DE, Hering BJ, Papas KK. Surgical protocol involving the infusion of paramagnetic microparticles for preferential incorporation within porcine islets. Transplant Proc. 2010 Dec;42(10):4209-12.

Kirchner VA, Suszynski TM, Radosevich DM, Humar A, Dunn TB, Hill MJ, Finger EB, Sutherland DER, Kandaswamy R. Anti-CD25 antibody (Daclizumab) maintenance therapy in pancreas transplantation. Transplant Proc. 2010; 42:2003-2005.
Scott WE III, Weegman BP, Ferrer-Fabrega J, Stein SA, Anazawa T, Kirchner VA, Rizzari MD, Stone J, Matsumoto S, Hammer BE, Balamurugan AN, Kidder LS, Suszynski TM, Avgoustiniatos ES, Stone SG, Tempelman LA, Sutherland DER, Hering BJ, Papas KK. Pancreas oxygen persufflation increases ATP levels as shown by nuclear magnetic resonance. Transplant Proc. 2010; 42:2011-2015.
Scott WE III, O'Brien TD, Ferrer-Fabrega J, Avgoustiniatos ES, Weegman BP, Anazawa T, Matsumoto S, Kirchner VA, Rizzari MD, Murtaugh MP, Suszynski TM, Aasheim T, Kidder LS, Hammer BE, Stone SG, Tempelman LA, Sutherland DER, Hering BJ, Papas KK. Persufflation improves pancreas preservation when compared with the two-layer method. Transplant Proc. 2010; 42: 2016-2019.
Weegman BP, Kirchner VA, Scott WE III, Avgoustiniatos ES, Suszynski TM, Ferrer-Fabrega J, Rizzari MD, Kidder LS, Kandaswamy R, Sutherland DER, Papas KK. Continuous real-time viability assessment of kidneys based on oxygen consumption. Transplant Proc. 2010; 42: 2020-2023.
Papas KK, Suszynski TM, Colton CK. Islet assessment for transplantation. Curr Opin Organ Transplant. 2009; 14:674-682.

Ferrer J, Scott WE 3rd, Weegman BP, Suszynski TM, Sutherland DE, Hering BJ, Papas KK. Pig pancreas anatomy: implications for pancreas procurement, preservation, and islet isolation. Transplantation. 2008; 86:1503-1510.

Suszynski TM, Wildey GM, Falde EJ, Cline GW, Maynard KS, Ko N, Sotiris J, Naji A, Hering BJ, Papas KK.The ATP/DNA Ratio Is a Better Indicator of Islet Cell Viability Than the ADP/ATP Ratio. Transplant Proc. 2008; 40:346-50.

Avgoustiniatos ES, Hering BJ, Rozak PR, Wilson JR, Tempelman LA, Balamurugan AN, Welch DP, Weegman BP, Suszynski TM, Papas KK. Commercially available gas-permeable cell culture bags may not prevent anoxia in cultured or shipped islets. Transplant Proc. 2008; 40:395-400.