Wade L. Schulz

     Email: schu1321@umn.edu

     Year Entered: 2006

     Degrees Received:
     University of Minnesota
     Genetics and Microbiology majors
     B.S.(2), 2006

     University of Minnesota
     Microbiology, Immunology and Cancer Biology
     Ph.D., 2012

     Honors and Awards:
     -University of Minnesota Undergraduate Research Opportunities
     Program, 2006
     -University of Minnesota Undergraduate Research Opportunities
     Program (UROP), 2005
     -American Society for Microbiology Undergraduate Research
Fellowship, 2005
-Best Undergraduate Poster, ASM North Central Branch Meeting, 2004
-University of Minnesota UROP, 2004

Thesis Advisor: Leslie Schiff, Ph.D.

Thesis Research: Cellular entry of reovirus.

Reovirus is currently undergoing stage III clinical trials as an oncolytic therapy. Prior work has shown that the ability of reovirus to efficiently enter transformed cells is a major determinant of its oncolytic ability, but little is known about how reovirus penetrates the limiting host cell membrane. Reovirus virions have been shown to undergo clathrin-mediated endocytosis, but it is unknown how infectious subviral particles (ISVPs), virions that have lost the outermost capsid protein through proteolysis, enter the cell. Through the use of multiple experimental approaches, we are investigating if ISVPs undergo endocytosis or whether they infect through direct penetration at the cell surface. In addition, we are using a genome-wide shRNA screen to identify host cell proteins that important for infection by reovirus particles in cell culture.

Publications (pubmed):

Schulz WL, Haj AK, Schiff LA. Reovirus Uses Multiple Endocytic Pathways for Cell Entry. J Virol. 2012 Dec;86(23):12665-75. 

Zacchi LF, Schulz WL, Davis DA. HOS2 and HDA1 encode histone deacetylases with opposing roles in Candida albicans morphogenesis. PLoS One. 2010 Aug 13;5(8):e12171.

Sandager MM, Nugent JL, Schulz WL, Messner RP, Tam PE. Interactions between multiple genetic determinants in the 5' UTR and VP1 capsid control pathogenesis of chronic post-viral myopathy caused by coxsackievirus B1. Virology 2008;372:35-47.