Daniel J. Beisang

     E-mail: beis0051@umn.edu

     Year Entered: 2007

     Degrees Received:
     University of Minnesota
     Physics major
     B.S., 2006

     Awards and Honors:
     -2003-2006 Dean's List, University of Minnesota
     -2004 Undergraduate Research Opportunities Program Grant
     -2006 Graduated Summa Cum Laude
     -National Heart, Lung, and Blood Institute and National Institute of
     General Medical Sciences partnership Service Award for
     Individual Predoctoral MD/PhD fellows, 2009-2012 

     Thesis Advisor: Paul Bohjanen M.D., Ph.D.

Thesis Research: mRNA decay is a crucial mechanism used by cells to control and alter their transcriptome.  We are interested in understanding the regulation of mRNA decay in the setting of T cell activation.  The Bohjanen lab has previously identified a conserved regulatory network controlled by the RNA-decay promoting protein CELF1.  My thesis research has focused on understanding the molecular targets and function of the CELF1 regulatory network.  We have shown that CELF1 controls a network of targets controlling the cellular pathways of cellular proliferation and apoptosis.  Furthermore, we have shown that the function of CELF1 is inhibited through an activation dependent phosphorylation event in T cells.  We are currently using high-throughput sequencing techniques to understand the impact of alternative polyadenylation on CELF1 mediated mRNA decay.

Publications (pubmed):

Beisang D, Rattenbacher B, Vlasova-St Louis IA, Bohjanen PR. Regulation of CUG-binding protein 1 (CUGBP1) binding to target transcripts upon T Cell activation. J Biol Chem. 2012 Jan 6;287(2):950-60.

Rattenbacher B, Beisang D, Wiesner DL, Jeschke JC, von Hohenberg M, St Louis-Vlasova IA, Bohjanen PR. Analysis of CUGBP1 targets identifies GU-repeat sequences that mediate rapid mRNA decay. Mol Cell Biol. 2010 Aug;30(16):3970-80.

Vlasova IA, Reilly C, Beisang D, Bohjanen PR. Control of T cell activation by AU-rich elements and GU-rich elements. RNA Society Meeting. 2009.

Beisang D, Rattenbacher B, Bohjanen PR. Identification of molecular targets of the RNA-binding protein CUGBP1 through computational sequence analysis. Bioinformatics: Building Bridges Sympsium. 2009.